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snu 387  (ATCC)


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    ATCC snu 387
    Snu 387, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 308 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snu 387/product/ATCC
    Average 96 stars, based on 308 article reviews
    snu 387 - by Bioz Stars, 2026-02
    96/100 stars

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    A, J33-4 MAIT TCR recognition of U266 cells incubated overnight with (inverse black triangles) or without (black circles) 5-OP-RU, in presence or not of E.hirae , E. faecalis , E. faecium , E. coli and B. longum (Left graph) and E. avium (right graph, independent experiment). TCR activation is measured by bioluminescence reading. B , different APC (human dendritic cells, THP1, melanoma MeWO and hepatocellular carcinoma <t>SNU387)</t> were tested for the synergistic effect of E faecalis onto activation of conventional MAIT TCR J33-4 in presence (inverse black triangles) or not (black circles) of 5-OP-RU. C , Flow cytometry analysis of MR1 surface membrane expression using the 26.5 anti MR1 antibody staining of U266, THP1 and dendritic cells (DC) incubated overnight without or with 5-OP-RU in presence or not of E.faecalis . D , Inhibition by Ac-6-FP (escalating doses from 0.2 to 200 uM) of recognition by MAIT TCR J33-4 of MR1 expressed on U266 cultured or not with 5-OP-RU (6.25 uM) in presence (triangles) or not (squares) of E.faecalis (MOI:125). E , MAIT TCR J33-4 activation by Myd88 -/- THP1 cells incubated overnight with (inverse black triangles) or without (black circles) 5-OP-RU (6.25 uM), in presence or not of E. faecalis (MOI:125). F , MAIT TCR J33-4 activation by U266 cells incubated overnight with (inverse black triangles) or without (black circles) 5-OP-RU (6.25 uM), in presence or not of E. faecalis (MOI:125) and E.faecalis derived outer membrane vesicles (OMV). G , Flow cytometry analysis of MR1 surface membrane expression using the 26.5 anti MR1 antibody staining of U266 incubated overnight without or with 5-OP-RU in presence or not of E. faecalis (MOI:125), compared to LPS (TLR4 ligand; 50ng/ml; left histogram), CPG (TLR9 ligand; 5uM, middle histogram) or LTA (TLR2 ligand; 30ug/ml co; right histogram). All data are shown as mean ± SEM with technical replicates represented by number of symbols in each graph. ****:P<0.0001, ***:0.0001<P<0.001, **:0.001<P<0.01, *:0.01<P<0.05, ns (non significant) by unpaired t-test (two-tailed).
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    https://www.bioz.com/result/snu387 cell lines/product/ATCC
    Average 96 stars, based on 1 article reviews
    snu387 cell lines - by Bioz Stars, 2026-02
    96/100 stars
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    snu 387 cells  (ATCC)
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    A, J33-4 MAIT TCR recognition of U266 cells incubated overnight with (inverse black triangles) or without (black circles) 5-OP-RU, in presence or not of E.hirae , E. faecalis , E. faecium , E. coli and B. longum (Left graph) and E. avium (right graph, independent experiment). TCR activation is measured by bioluminescence reading. B , different APC (human dendritic cells, THP1, melanoma MeWO and hepatocellular carcinoma <t>SNU387)</t> were tested for the synergistic effect of E faecalis onto activation of conventional MAIT TCR J33-4 in presence (inverse black triangles) or not (black circles) of 5-OP-RU. C , Flow cytometry analysis of MR1 surface membrane expression using the 26.5 anti MR1 antibody staining of U266, THP1 and dendritic cells (DC) incubated overnight without or with 5-OP-RU in presence or not of E.faecalis . D , Inhibition by Ac-6-FP (escalating doses from 0.2 to 200 uM) of recognition by MAIT TCR J33-4 of MR1 expressed on U266 cultured or not with 5-OP-RU (6.25 uM) in presence (triangles) or not (squares) of E.faecalis (MOI:125). E , MAIT TCR J33-4 activation by Myd88 -/- THP1 cells incubated overnight with (inverse black triangles) or without (black circles) 5-OP-RU (6.25 uM), in presence or not of E. faecalis (MOI:125). F , MAIT TCR J33-4 activation by U266 cells incubated overnight with (inverse black triangles) or without (black circles) 5-OP-RU (6.25 uM), in presence or not of E. faecalis (MOI:125) and E.faecalis derived outer membrane vesicles (OMV). G , Flow cytometry analysis of MR1 surface membrane expression using the 26.5 anti MR1 antibody staining of U266 incubated overnight without or with 5-OP-RU in presence or not of E. faecalis (MOI:125), compared to LPS (TLR4 ligand; 50ng/ml; left histogram), CPG (TLR9 ligand; 5uM, middle histogram) or LTA (TLR2 ligand; 30ug/ml co; right histogram). All data are shown as mean ± SEM with technical replicates represented by number of symbols in each graph. ****:P<0.0001, ***:0.0001<P<0.001, **:0.001<P<0.01, *:0.01<P<0.05, ns (non significant) by unpaired t-test (two-tailed).
    Snu 387 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/snu 387 cells/product/ATCC
    Average 96 stars, based on 1 article reviews
    snu 387 cells - by Bioz Stars, 2026-02
    96/100 stars
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    A, J33-4 MAIT TCR recognition of U266 cells incubated overnight with (inverse black triangles) or without (black circles) 5-OP-RU, in presence or not of E.hirae , E. faecalis , E. faecium , E. coli and B. longum (Left graph) and E. avium (right graph, independent experiment). TCR activation is measured by bioluminescence reading. B , different APC (human dendritic cells, THP1, melanoma MeWO and hepatocellular carcinoma SNU387) were tested for the synergistic effect of E faecalis onto activation of conventional MAIT TCR J33-4 in presence (inverse black triangles) or not (black circles) of 5-OP-RU. C , Flow cytometry analysis of MR1 surface membrane expression using the 26.5 anti MR1 antibody staining of U266, THP1 and dendritic cells (DC) incubated overnight without or with 5-OP-RU in presence or not of E.faecalis . D , Inhibition by Ac-6-FP (escalating doses from 0.2 to 200 uM) of recognition by MAIT TCR J33-4 of MR1 expressed on U266 cultured or not with 5-OP-RU (6.25 uM) in presence (triangles) or not (squares) of E.faecalis (MOI:125). E , MAIT TCR J33-4 activation by Myd88 -/- THP1 cells incubated overnight with (inverse black triangles) or without (black circles) 5-OP-RU (6.25 uM), in presence or not of E. faecalis (MOI:125). F , MAIT TCR J33-4 activation by U266 cells incubated overnight with (inverse black triangles) or without (black circles) 5-OP-RU (6.25 uM), in presence or not of E. faecalis (MOI:125) and E.faecalis derived outer membrane vesicles (OMV). G , Flow cytometry analysis of MR1 surface membrane expression using the 26.5 anti MR1 antibody staining of U266 incubated overnight without or with 5-OP-RU in presence or not of E. faecalis (MOI:125), compared to LPS (TLR4 ligand; 50ng/ml; left histogram), CPG (TLR9 ligand; 5uM, middle histogram) or LTA (TLR2 ligand; 30ug/ml co; right histogram). All data are shown as mean ± SEM with technical replicates represented by number of symbols in each graph. ****:P<0.0001, ***:0.0001<P<0.001, **:0.001<P<0.01, *:0.01<P<0.05, ns (non significant) by unpaired t-test (two-tailed).

    Journal: bioRxiv

    Article Title: Synergistic role of riboflavin-auxotrophic Enterococcus for MR1 expression and intra-tumoral mucosal associated invariant T (MAIT) cell activation

    doi: 10.1101/2025.11.25.690539

    Figure Lengend Snippet: A, J33-4 MAIT TCR recognition of U266 cells incubated overnight with (inverse black triangles) or without (black circles) 5-OP-RU, in presence or not of E.hirae , E. faecalis , E. faecium , E. coli and B. longum (Left graph) and E. avium (right graph, independent experiment). TCR activation is measured by bioluminescence reading. B , different APC (human dendritic cells, THP1, melanoma MeWO and hepatocellular carcinoma SNU387) were tested for the synergistic effect of E faecalis onto activation of conventional MAIT TCR J33-4 in presence (inverse black triangles) or not (black circles) of 5-OP-RU. C , Flow cytometry analysis of MR1 surface membrane expression using the 26.5 anti MR1 antibody staining of U266, THP1 and dendritic cells (DC) incubated overnight without or with 5-OP-RU in presence or not of E.faecalis . D , Inhibition by Ac-6-FP (escalating doses from 0.2 to 200 uM) of recognition by MAIT TCR J33-4 of MR1 expressed on U266 cultured or not with 5-OP-RU (6.25 uM) in presence (triangles) or not (squares) of E.faecalis (MOI:125). E , MAIT TCR J33-4 activation by Myd88 -/- THP1 cells incubated overnight with (inverse black triangles) or without (black circles) 5-OP-RU (6.25 uM), in presence or not of E. faecalis (MOI:125). F , MAIT TCR J33-4 activation by U266 cells incubated overnight with (inverse black triangles) or without (black circles) 5-OP-RU (6.25 uM), in presence or not of E. faecalis (MOI:125) and E.faecalis derived outer membrane vesicles (OMV). G , Flow cytometry analysis of MR1 surface membrane expression using the 26.5 anti MR1 antibody staining of U266 incubated overnight without or with 5-OP-RU in presence or not of E. faecalis (MOI:125), compared to LPS (TLR4 ligand; 50ng/ml; left histogram), CPG (TLR9 ligand; 5uM, middle histogram) or LTA (TLR2 ligand; 30ug/ml co; right histogram). All data are shown as mean ± SEM with technical replicates represented by number of symbols in each graph. ****:P<0.0001, ***:0.0001

    Article Snippet: MeWo and SNU387 cell lines were kindly provided by Dr. Scott Wilson’s lab and cul-tured according to ATCC protocol.

    Techniques: Incubation, Activation Assay, Flow Cytometry, Membrane, Expressing, Staining, Inhibition, Cell Culture, Derivative Assay, Two Tailed Test